Presentation Institutes: UMCG

1. Contact information and URL
Dr. F. Stellaard:                        f.stellaard@med.umcg.nl,         Tel.: +31 50 3613108
Dr. D.J. Reijngoud:       d.j.reijngoud@med.umcg.nl,     Tel.: +31 50 3611253
Theo van Dijk:             t.h.van.dijk@lc.umcg.nl,           Tel.: +31 50 3611732
Theo Boer:                   t.boer@lc.umcg.nl,                   Tel: +31 50 3612424
Elles Jonkers:              e.z.jonkers@lc.umcg.nl,            Tel: +31 50 3619558

University Medical Center Groningen
Center for Liver, Digestive and Metabolic diseases (CLDS)
Laboratory of Medicine and Laboratory of Pediatrics
Hanzeplein 1, 9713 GZ Groningen, The Netherlands
PO box 30.001, 9700 RB Groningen, The Netherlands

URLS
www.labpediatricsrug.nl

Stable isotope equipment

An Agilent 6890 GC with combustion interface (GC/C combustion III) coupled to a Finnigan DeltaplusXP
A Gilson autosampler and coupled to a Finnigan Delta S
A 5975C quadrupole GC/MS
A Perkin Elmer 200 series LC coupled to an Applied Biosystems API 3000 MS/MS

Research themes
CLDS research interests are centered around chronic metabolic diseases:

Arteriosclerosis (cholesterol and bile acid metabolism);
Diabetes Mellitus (glucose, lipid, bile acid metabolism);

and research projects are performed in men, rats, various mouse models and cell cultures

Stable isotope analyses

Compound specific 13C and 2H analysis in bile, blood, urine, feces related to cholesterol, bile acids, glucose, palmitic acid, stearic acid, oleic acid<
Overall isotope enrichment using IRMS, specific isotope enrichments using GC/MS
Primary applications: Measurement of pool sizes and synthesis rates of bile acids, synthesis rates and absorption rates of cholesterol, synthesis rates of fatty acids, digestion of lipids and carbohydrates, glucose absorption and hepatic glucose fluxes