Archieven

Academic Medical Center Amsterdam – Clinical Chemistry / Woman and Child / Endocrinology

PO Box 22660, 1100DD Amsterdam / Meibergdreef 9, 1100AZ Amsterdam, NL

Contact:
Henk Schierbeek
h.schierbeek@amc.uva.nl
31(0)205666299

Research line
Health and Disease

Topics
metabolic kinetics
amino acids, peptides, carbohydrates, protein synthesis, energy expenditure
neonates, adults, laboratory animals: mouse, rat, pig, baboon
insulin resistance, lungs (surfactant), gut, oxidative stress and malnutrition

Isotopes
¹³C, ¹⁵N, ¹⁸O, ²H

Instruments
LC/MS/MS, GC/MS/MS, GC/C/IRMS, EA/C/IRMS, SIRIS, GC/MS, LC/C/IRMS

LCMS\MS, Waters, Premier, Triple quad
LCMS\MS, Waters, Xevo, Triple quad
LCMS\MS, Thermo Fisher, Velos pro, Linear Trap
GCMS\MS, Agilent 7000 GC\QQQ, Triple quad
GCMS, Agilent 5975C, Single quad
GCMS, Agilent 5973, Single quad
GCMS, Agilent 5973, Single quad
IRMS, Thermo Fisher, Delta plus
– GC/C/IRMS (+ Headspace)
IRMS, Thermo Fisher, Delta XP plus
– GC/C/IRMS
– LC/C/IRMS
– TCEA/IRMS
– Con Flow EA/IRMS
Infrared Spectrometer, Fisher, Helifan, CO₂ analysis

Amsterdam University – Institute for Biodiversity and Ecosystem Dynamics (IBED)

PO Box 94240, 1090GE Amsterdam
Science Park 904, 1098 XH Amsterdam, NL

Contact
Prof. Karsten kalbitz
K.Kalbitz@uva.nl
31(0)205257434/6637

Cerli Chiara – c.cerli@uva.nl

Research line
Ecology

Topics
With the analyses of C, N, H, and S stable isotopes (δ¹³C, δ¹⁸O, δ¹⁵N, δ²H, δ³⁴S) in environmental samples, from soils to sediment and water we study:
–         Effects of changing environmental conditions on the mechanisms and rates of C stabilization in soils
–         Interactions between land degradation and C sequestration in soils
–         Reconstruction of past environments and climates to improve future predictions
–         Fate of organic pollutants in soils, sediments and water and interactions with organisms exposed to pollution
–         Application of CSIA in forensic science

Isotopes
13C, 15N, 18O, 2H, 32S

Instruments
EA/C/IRMS, EA/P/IRMS, GC/MS, TOC/IRMS

Stable Isotope Ratio Mass Spectrometer IsoPrime 100 (Elementar – Isoprime) connected with:

Elemental Analyzer, vario ISOTOPE cube
·        EA combustion [CO₂ (δ¹³C, δ¹⁸O), N2 (δ¹⁵N), H2 (²H), SO₂ (δ³⁴S)]
·        EA pyrolysis (solid) [H₂ (²H), CO (δ¹⁸O)]
·        EA pyrolysis (liquid) [H₂ (²H), CO (δ¹⁸O)]

GC/MS, Agilent 7890A GC-MS-FID with N2 cooled PTV injector, GC5 interface
· [CO₂ (δ¹³C), N₂ (δ¹⁵N), H₂ (δ²H), CO (δ¹⁸O)]

Gas concentrator
· CO₂ and CH₄ (δ¹³C), N₂O (δ¹⁵N),

High temperature combustion TOC analyzer, TOC cube, TOC cube interface
· [CO₂ (δ¹³C), N₂ (δ¹⁵N)]
CO₂ (δ¹³C, δ¹⁸O), N₂ (δ¹⁵N), H₂ (²H), SO₂ (δ³⁴S)

Groningen University Medical Center – Center for Liver, Digestive and Metabolic diseases

PO Box 30.001, 9700 RB Groningen
Hanzeplein 1, 9713GZ Groningen, NL

Contact
Bert Groen
a.k.groen@umcg.nl
31(0)503632669

Research line
Health and Disease

Topics
Arteriosclerosis (cholesterol and bile acid metabolism)
Diabetes Mellitus (glucose, lipid metabolism)
men, rats, various mouse models and cell cultures
regulation of metabolic pathways
kinetics of metabolic pathways applying tracer technology
colon delivery device
cholesterol, bile acids, glucose, palmitic acid, stearic acid, oleic acid, urea, bicarbonate, breath CO₂kinetic studies in mouse blood, requiring frequent sampling of minimal blood spots

Isotopes
¹³C, ¹⁵N, ²H

Instruments
GC/C/IRMS, GC/P/IRMS, GC/MS, LC/MS/MS

Agilent 6890 GC/ Finnigan DeltaplusXL IRMS with CTC Pal autosampler
Two Agilent 6890 GC/ 5975 quadrupole MS with CTC Pal auto sampler
Perkin ElmerLC/API 3000 MS/MS with Perkin Elmer auto sampler
Shimadzu LC/API 3000 MS/MS with Shimadzu auto sampler

Maastricht University – Human biology, Movement Sciences

PO Box 616, 6200 MD Maastricht
Universiteitssingel 50, 6229 ER Maastricht, NL

Contact
Luc van Loon
L.vanLoon@maastrichtuniversity.nl
31(0)433881397 /31(0)613260552

Ellen Blaak
e.blaak@maastrichtuniversity.nl

http://www.m3-research.nl
http://www.sirc.unimaas.nl

Research line
Health and Disease

Topics
protein, carbohydrate and fat and metabolism in vivo in humans
(nutritional) intervention studies
to assess substrate fluxes in different physiological situations and/or patient groups.

Isotopes
¹³C, ¹⁵N, ²H

Instruments
GC/C/IRMS, GC/MS

·        Thermo Scientific MAT-253 GC combustion IRMS
·        Agilent GC-MS (GC:7870A; MS:5975C) (Electron Impact mode)
·        Agilent GC-MS (GC: 6890N; MS: 5973) (EI- and CI-mode)
·        Finnigan DELTAplus GC combustion IRMS with detectors for CO₂ and N₂.

GC-C-IRMS:
·        Enrichment of breath CO₂·        Concentration and enrichment of CO2 in blood samples
·        Deuterated water enrichment
·        Enrichment of ¹³C-labeled glucose and fatty acids in plasma samples and muscle extracts
·        Enrichment of ¹³C-labeled fatty acids in plasma FFA and plasma triglycerides and in the different lipid fractions of muscle
·        Enrichment of ¹³C- and ¹⁵N-labeled amino acids in plasma samples, in mixed muscle protein and albumin samples.

GC-MS:
·        Plasma enrichment of [²H₅]glycerol, [U-¹³C]glycerol, [1-¹³C]KIC, [6,6-²H₂]glucose,
[²H₂]palmitate (enrichment in plasma FFA and triglycerides), [²H5-ring]phenylalanine, [1-¹³C]phenylalanine, [1-¹³C]leucine,
[1-¹³C]tyrosine [2H₂]tyrosine and [2H₄]tyrosine,
·        Concentrations of phenylalanine, tyrosine and leucine
·        [²H₂]tyrosine
·        [U¹³C]phenylalanine
·        [²H₅]phenylalanine
·        Intrinsically [1-¹³C]phenylalanine and [1-¹³C]leucine labeled milk protein
·        [²H₂]glucose
·        [U¹³C]glucose
·        [²H₂]palmitate
·        [U¹³C]palmitate
·        [²H₅]-glycerol

Research Institute for Chromatography – Research & Development

Pres. Kennedypark 26
8500 Kortrijk, Belgium

Contact
Emmie Dumont
emmied@richrom.com
32(0)56204031

Research line
Analytical development

Topics
optimization of the chromatographic separation before IRMS
implementation of 2D-GC and 2D-LC
complex natural samples.

Isotopes
¹³C, ¹⁵N, ²H

Instruments
GC/Py/IRMS, 2D-GC/Py/IRMS, LC/C/IRMS

GC-C/Py-IRMS (Agilent GC 7890 + Thermo delta V advantage)
2D-GC-C/Py-IRMS
LC-C-IRMS (All Thermo)

Taal: