Maastricht University – Human biology, Movement Sciences

Maastricht University – Human biology, Movement Sciences

PO Box 616, 6200 MD Maastricht
Universiteitssingel 50, 6229 ER Maastricht, NL

Luc van Loon
31(0)433881397 /31(0)613260552

Ellen Blaak

Research line
Health and Disease

protein, carbohydrate and fat and metabolism in vivo in humans
(nutritional) intervention studies
to assess substrate fluxes in different physiological situations and/or patient groups.

13C, 15N, 2H


·        Thermo Scientific MAT-253 GC combustion IRMS
·        Agilent GC-MS (GC:7870A; MS:5975C) (Electron Impact mode)
·        Agilent GC-MS (GC: 6890N; MS: 5973) (EI- and CI-mode)
·        Finnigan DELTAplus GC combustion IRMS with detectors for CO2 and N2.

·        Enrichment of breath CO2
·        Concentration and enrichment of CO2 in blood samples
·        Deuterated water enrichment
·        Enrichment of 13C-labeled glucose and fatty acids in plasma samples and muscle     extracts
·        Enrichment of 13C-labeled fatty acids in plasma FFA and plasma triglycerides and in the different lipid fractions of muscle
·        Enrichment of 13C- and 15N-labeled amino acids in plasma samples, in mixed muscle protein and albumin samples.

·        Plasma enrichment of [2H5]glycerol, [U-13C]glycerol, [1-13C]KIC, [6,6-2H2]glucose,
[2H2]palmitate (enrichment in plasma FFA and triglycerides), [2H5-ring]phenylalanine, [1-13C]phenylalanine, [1-13C]leucine,
[1-13C]tyrosine [2H2]tyrosine and [2H4]tyrosine,
·        Concentrations of phenylalanine, tyrosine and leucine
·        [2H2]tyrosine
·        [U13C]phenylalanine
·        [2H5]phenylalanine
·        Intrinsically [1-13C]phenylalanine and [1-13C]leucine labeled milk protein
·        [2H2]glucose
·        [U13C]glucose
·        [2H2]palmitate
·        [U13C]palmitate
·        [2H5]-glycerol