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Academic Medical Center Amsterdam – Clinical Chemistry / Woman and Child / Endocrinology

Academic Medical Center Amsterdam – Clinical Chemistry /  Woman and Child / Endocrinology

PO Box 22660, 1100DD Amsterdam / Meibergdreef 9, 1100AZ Amsterdam, NL

Contact:
Henk Schierbeek
h.schierbeek@amc.uva.nl
31(0)205666299

Research line
Health and Disease

Topics
metabolic kinetics
amino acids, peptides, carbohydrates, protein synthesis, energy expenditure
neonates, adults, laboratory animals: mouse, rat, pig, baboon
insulin resistance, lungs (surfactant), gut, oxidative stress and malnutrition

Isotopes
13C, 15N, 18O, 2H

Instruments
LC/MS/MS, GC/MS/MS, GC/C/IRMS, EA/C/IRMS, SIRIS, GC/MS, LC/C/IRMS

LCMS\MS, Waters, Premier, Triple quad
LCMS\MS, Waters, Xevo, Triple quad
LCMS\MS, Thermo Fisher, Velos pro, Linear Trap
GCMS\MS, Agilent 7000 GC\QQQ, Triple quad
GCMS, Agilent 5975C, Single quad
GCMS, Agilent 5973, Single quad
GCMS, Agilent 5973, Single quad
IRMS, Thermo Fisher, Delta plus
– GC/C/IRMS (+ Headspace)
IRMS, Thermo Fisher, Delta XP plus
– GC/C/IRMS
– LC/C/IRMS
– TCEA/IRMS
– Con Flow EA/IRMS
Infrared Spectrometer, Fisher, Helifan, CO2 analysis

Amsterdam University – Institute for Biodiversity and Ecosystem Dynamics (IBED)

Amsterdam University – Institute for Biodiversity and Ecosystem Dynamics (IBED)

PO Box 94240, 1090GE Amsterdam
Science Park 904, 1098 XH Amsterdam, NL

Contact
Prof. Karsten kalbitz
K.Kalbitz@uva.nl
31(0)205257434/6637

Cerli Chiara – c.cerli@uva.nl

Research line
Ecology

Topics
With the analyses of C, N, H, and S stable isotopes (δ13C, δ18O, δ15N, δ2H, δ34S) in environmental samples, from soils to sediment and water we study:
–         Effects of changing environmental conditions on the mechanisms and rates of C      stabilization in soils
–         Interactions between land degradation and C sequestration in soils
–         Reconstruction of past environments and climates to improve future predictions
–         Fate of organic pollutants in soils, sediments and water and interactions with organisms exposed to pollution
–         Application of CSIA in forensic science

Isotopes
13C, 15N, 18O, 2H,  32S

Instruments
EA/C/IRMS, EA/P/IRMS, GC/MS, TOC/IRMS

Stable Isotope Ratio Mass Spectrometer IsoPrime 100 (Elementar – Isoprime) connected with:

Elemental Analyzer, vario ISOTOPE cube
·        EA combustion [CO213C, δ18O), N2 (δ15N), H2 (2H), SO234S)]
·        EA pyrolysis (solid) [H2 (2H), CO (δ18O)]
·        EA pyrolysis (liquid) [H2 (2H), CO (δ18O)]

GC/MS, Agilent 7890A GC-MS-FID with N2 cooled PTV injector, GC5 interface
·        [CO213C), N215N), H22H), CO (δ18O)]

Gas concentrator
·        CO2 and CH413C), N2O (δ15N),

High temperature combustion TOC analyzer, TOC cube, TOC cube interface
·        [CO213C), N215N)]
CO213C, δ18O), N215N), H2 (2H), SO234S)

Groningen University Medical Center – Center for Liver, Digestive and Metabolic diseases

Groningen University Medical Center – Center for Liver, Digestive and Metabolic diseases

PO Box 30.001, 9700 RB Groningen
Hanzeplein 1, 9713GZ Groningen, NL

Contact
Bert Groen
a.k.groen@umcg.nl
31(0)503632669

Research line
Health and Disease

Topics
Arteriosclerosis (cholesterol and bile acid metabolism)
Diabetes Mellitus (glucose, lipid metabolism)
men, rats, various mouse models and cell cultures
regulation of metabolic pathways
kinetics of metabolic pathways applying tracer technology
colon delivery device
cholesterol, bile acids, glucose, palmitic acid, stearic acid, oleic acid, urea, bicarbonate, breath CO2
kinetic studies in mouse blood, requiring frequent sampling of minimal blood spots

Isotopes
13C, 15N, 2H

Instruments
GC/C/IRMS, GC/P/IRMS, GC/MS, LC/MS/MS

Agilent 6890 GC/ Finnigan DeltaplusXL IRMS with CTC Pal autosampler
Two Agilent 6890 GC/ 5975 quadrupole MS with CTC Pal auto sampler
Perkin ElmerLC/API 3000 MS/MS with Perkin Elmer auto sampler
Shimadzu LC/API 3000 MS/MS with Shimadzu auto sampler

Maastricht University – Human biology, Movement Sciences

Maastricht University – Human biology, Movement Sciences

PO Box 616, 6200 MD Maastricht
Universiteitssingel 50, 6229 ER Maastricht, NL

Contact
Luc van Loon
L.vanLoon@maastrichtuniversity.nl
31(0)433881397 /31(0)613260552

Ellen Blaak
e.blaak@maastrichtuniversity.nl

http://www.m3-research.nl
http://www.sirc.unimaas.nl

Research line
Health and Disease

Topics
protein, carbohydrate and fat and metabolism in vivo in humans
(nutritional) intervention studies
to assess substrate fluxes in different physiological situations and/or patient groups.

Isotopes
13C, 15N, 2H

Instruments
GC/C/IRMS, GC/MS

·        Thermo Scientific MAT-253 GC combustion IRMS
·        Agilent GC-MS (GC:7870A; MS:5975C) (Electron Impact mode)
·        Agilent GC-MS (GC: 6890N; MS: 5973) (EI- and CI-mode)
·        Finnigan DELTAplus GC combustion IRMS with detectors for CO2 and N2.

GC-C-IRMS:
·        Enrichment of breath CO2
·        Concentration and enrichment of CO2 in blood samples
·        Deuterated water enrichment
·        Enrichment of 13C-labeled glucose and fatty acids in plasma samples and muscle     extracts
·        Enrichment of 13C-labeled fatty acids in plasma FFA and plasma triglycerides and in the different lipid fractions of muscle
·        Enrichment of 13C- and 15N-labeled amino acids in plasma samples, in mixed muscle protein and albumin samples.

GC-MS:
·        Plasma enrichment of [2H5]glycerol, [U-13C]glycerol, [1-13C]KIC, [6,6-2H2]glucose,
[2H2]palmitate (enrichment in plasma FFA and triglycerides), [2H5-ring]phenylalanine, [1-13C]phenylalanine, [1-13C]leucine,
[1-13C]tyrosine [2H2]tyrosine and [2H4]tyrosine,
·        Concentrations of phenylalanine, tyrosine and leucine
·        [2H2]tyrosine
·        [U13C]phenylalanine
·        [2H5]phenylalanine
·        Intrinsically [1-13C]phenylalanine and [1-13C]leucine labeled milk protein
·        [2H2]glucose
·        [U13C]glucose
·        [2H2]palmitate
·        [U13C]palmitate
·        [2H5]-glycerol

Research Institute for Chromatography – Research & Development

Research Institute for Chromatography – Research & Development

Pres. Kennedypark 26
8500 Kortrijk, Belgium

Contact
Emmie Dumont
emmied@richrom.com
32(0)56204031

Research line
Analytical development

Topics
optimization of the chromatographic separation before IRMS
implementation of 2D-GC and 2D-LC
complex natural samples.

Isotopes
13C, 15N, 2H

Instruments
GC/Py/IRMS, 2D-GC/Py/IRMS, LC/C/IRMS

GC-C/Py-IRMS (Agilent GC 7890 + Thermo delta V advantage)
2D-GC-C/Py-IRMS
LC-C-IRMS (All Thermo)

Utrecht University – Earth Sciences

Utrecht University – Earth Sciences

PO Box 80.021, 3508 TA Utrecht
Princetonlaan 8, 3584 CB Utrecht, NL

Contact
Michiel Kienhuis
m.v.m.kienhuis@uu.nl
31(0) 6 37338646

Research line
Ecology, Geology, Microbiology, Paleoclimatology

Topics
Stratigraphy & paleontology : astronomical timescales and paleoclimate
Marine Geochemistry and Chemical Oceanography: marine sediments /overlying water column, eastern Mediterranean.
Organic Geochemistry: the natural environment (i.e. water, soil, sediments), rocks and fossil fuels.
Experimental Biogeochemistry: environmental relevant processes under controlled conditions.
Geochemical Modeling: improving the quantitative understanding of the cycling of bioactive elements.
Petrology group: stable isotope biomarkers for early life. Volcanism crater lakes and natural pollution.
Paleoecology
Biomarine Sciences: studying Paleoclimate

Isotopes
13C, 15N, 18O, 2H, 32S, 37Cl, Clumped oxygen isotopes (and others, like 29Si, 44Ca and heavier elements with nanoSIMS)

Instruments
DI-IRMS ISOCARB, DI-IRMS Kiel, EA/C/IRMS, TOC/IRMS, GC/C/IRMS, LA/IRMS, GC/IRMS, nanoSIMS

VG SIRA-24 EM, a dual inlet machine equipped with an ISOCARB.
Thermo Finnigan MAT with Kiel-III Device.
Thermo DeltaV with isolink Elemental Analyser and/or TCEA.
Thermo DeltaplusXP with GC-C-III.
Thermo Delta-V with laser-ablation/GC-C-III in development.
Thermo Delta-V with either a GasBench-II or a GC-C-III.
Ordered: Thermo MAT253 plus with a Kiel-IV for clumped carbonate isotopes.
Cameca NanoSIMS 50L for multi elemental and stable isotope ratio measurements and imaging.
A set of vacuum lines for offline preparation of samples.