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Academic Medical Center Amsterdam – Clinical Chemistry / Woman and Child / Endocrinology

Academic Medical Center Amsterdam – Clinical Chemistry /  Woman and Child / Endocrinology

PO Box 22660, 1100DD Amsterdam / Meibergdreef 9, 1100AZ Amsterdam, NL

Contact:
Henk Schierbeek
h.schierbeek@amc.uva.nl
31(0)205666299

Research line
Health and Disease

Topics
metabolic kinetics
amino acids, peptides, carbohydrates, protein synthesis, energy expenditure
neonates, adults, laboratory animals: mouse, rat, pig, baboon
insulin resistance, lungs (surfactant), gut, oxidative stress and malnutrition

Isotopes
13C, 15N, 18O, 2H

Instruments
LC/MS/MS, GC/MS/MS, GC/C/IRMS, EA/C/IRMS, SIRIS, GC/MS, LC/C/IRMS

LCMS\MS, Waters, Premier, Triple quad
LCMS\MS, Waters, Xevo, Triple quad
LCMS\MS, Thermo Fisher, Velos pro, Linear Trap
GCMS\MS, Agilent 7000 GC\QQQ, Triple quad
GCMS, Agilent 5975C, Single quad
GCMS, Agilent 5973, Single quad
GCMS, Agilent 5973, Single quad
IRMS, Thermo Fisher, Delta plus
– GC/C/IRMS (+ Headspace)
IRMS, Thermo Fisher, Delta XP plus
– GC/C/IRMS
– LC/C/IRMS
– TCEA/IRMS
– Con Flow EA/IRMS
Infrared Spectrometer, Fisher, Helifan, CO2 analysis

Antwerp University Hospital – Gastroenterology and Hepatology

Antwerp University Hospital –   Gastroenterology and Hepatology

Wilrijkstraat 10, 2650 Edegem, B

Contact
Paul Pelckmans
paul.pelckmans@uza.be
32(0)38213323

Research line
Health and Disease

Topics
13C breath tests
urea – 13C
mixed triglyceride – 13C
octanoic acid – 13C
d-glucose u –13C6
aminopyrine – 13C2
lactose – 13C

Isotopes
13C

Instruments
GC/IRMS

IRMS Sercon ABCA V

Groningen University Medical Center – Center for Liver, Digestive and Metabolic diseases

Groningen University Medical Center – Center for Liver, Digestive and Metabolic diseases

PO Box 30.001, 9700 RB Groningen
Hanzeplein 1, 9713GZ Groningen, NL

Contact
Bert Groen
a.k.groen@umcg.nl
31(0)503632669

Research line
Health and Disease

Topics
Arteriosclerosis (cholesterol and bile acid metabolism)
Diabetes Mellitus (glucose, lipid metabolism)
men, rats, various mouse models and cell cultures
regulation of metabolic pathways
kinetics of metabolic pathways applying tracer technology
colon delivery device
cholesterol, bile acids, glucose, palmitic acid, stearic acid, oleic acid, urea, bicarbonate, breath CO2
kinetic studies in mouse blood, requiring frequent sampling of minimal blood spots

Isotopes
13C, 15N, 2H

Instruments
GC/C/IRMS, GC/P/IRMS, GC/MS, LC/MS/MS

Agilent 6890 GC/ Finnigan DeltaplusXL IRMS with CTC Pal autosampler
Two Agilent 6890 GC/ 5975 quadrupole MS with CTC Pal auto sampler
Perkin ElmerLC/API 3000 MS/MS with Perkin Elmer auto sampler
Shimadzu LC/API 3000 MS/MS with Shimadzu auto sampler

KU Leuven – Department of Clinical and Experimental Medicine

KU Leuven – Department of Clinical and Experimental Medicine

O&N1, box 701 / Herestraat 49, 3000 Leuven, B

Contact
Prof. Kristin Verbeke
kristin.verbeke@med.kuleuven.be
32(0)16330150

Research line
Health and disease

Topics
Estimation of colonic short chain fatty acid production
Analysis of colonic ammonia metabolism using 15N-labelled lactose ureide
Breath tests using 13C-labelled substrates

Isotopes
13C, 15N

Instruments
GC/IRMS, EA/C/IRMS, GC/C/IRMS

ABCA (Sercon, UK)
ANCA (Sercon, UK)
GC-C-IRMS (Thermo)

Maastricht University – Human biology, Movement Sciences

Maastricht University – Human biology, Movement Sciences

PO Box 616, 6200 MD Maastricht
Universiteitssingel 50, 6229 ER Maastricht, NL

Contact
Luc van Loon
L.vanLoon@maastrichtuniversity.nl
31(0)433881397 /31(0)613260552

Ellen Blaak
e.blaak@maastrichtuniversity.nl

http://www.m3-research.nl
http://www.sirc.unimaas.nl

Research line
Health and Disease

Topics
protein, carbohydrate and fat and metabolism in vivo in humans
(nutritional) intervention studies
to assess substrate fluxes in different physiological situations and/or patient groups.

Isotopes
13C, 15N, 2H

Instruments
GC/C/IRMS, GC/MS

·        Thermo Scientific MAT-253 GC combustion IRMS
·        Agilent GC-MS (GC:7870A; MS:5975C) (Electron Impact mode)
·        Agilent GC-MS (GC: 6890N; MS: 5973) (EI- and CI-mode)
·        Finnigan DELTAplus GC combustion IRMS with detectors for CO2 and N2.

GC-C-IRMS:
·        Enrichment of breath CO2
·        Concentration and enrichment of CO2 in blood samples
·        Deuterated water enrichment
·        Enrichment of 13C-labeled glucose and fatty acids in plasma samples and muscle     extracts
·        Enrichment of 13C-labeled fatty acids in plasma FFA and plasma triglycerides and in the different lipid fractions of muscle
·        Enrichment of 13C- and 15N-labeled amino acids in plasma samples, in mixed muscle protein and albumin samples.

GC-MS:
·        Plasma enrichment of [2H5]glycerol, [U-13C]glycerol, [1-13C]KIC, [6,6-2H2]glucose,
[2H2]palmitate (enrichment in plasma FFA and triglycerides), [2H5-ring]phenylalanine, [1-13C]phenylalanine, [1-13C]leucine,
[1-13C]tyrosine [2H2]tyrosine and [2H4]tyrosine,
·        Concentrations of phenylalanine, tyrosine and leucine
·        [2H2]tyrosine
·        [U13C]phenylalanine
·        [2H5]phenylalanine
·        Intrinsically [1-13C]phenylalanine and [1-13C]leucine labeled milk protein
·        [2H2]glucose
·        [U13C]glucose
·        [2H2]palmitate
·        [U13C]palmitate
·        [2H5]-glycerol

Wageningen University – Animal Nutrition Group

Wageningen University – Animal Nutrition Group

PO Box 338,6700 AH Wageningen
De Elst 1, 6708 WD Wageningen,NL                                                                                            

Contact
Joost van den Borne
joost.vandenborne@wur.nl
31(0)317482912

Research line
Health and Disease

Topics
Measuring post-absorptive processes in animals, e.g. nutrient oxidation, nutrient fluxes (such as entry rates, turnover and nutrient cycling). Nutrients of study include glucose, amino acids, urea, short chain fatty acids, long chain fatty acids and lactate
Measuring digestive processes, incl. gastric emptying, passage rates of labeled ingredients,
urea recycling (ruminants), uric acid recycling (poultry), absorption rates of short chain fatty acids (ruminants), fermentation.

Isotopes
13C, 15N

Instruments
GC/C/IRMS, SIRIS, EA/C/IRMS